After the introduction of better alkali-and-builder-stable proteases from the 1960s onwards, the use of enzymes in detergents gained prominence. The detergent did not perform well due to insufficient activity and poor stability of trypsin with other constituents of the detergent. This detergent had the enzyme trypsin that had been extracted from the pancreas of pigs. “Burnus”, the first detergent enzyme was patented and sold in Germany in 1914. Multi enzyme detergents have even replaced about 25% of the laundry detergent surfactants without any losses in cleaning performance. These blends make the enzymes more tolerant of the ingredients in the detergent that include surfactants, builders, bleaching chemicals, and alkaline salts. Most of these detergents are created with a mix of 2 or more enzymes and can go up to 8 different enzymes in a single formulation. Other than efficient cleaning, using enzymes in detergents are sustainable and also save energy as they function in low temperatures and do not require heated water. Enzymes are used in biological detergents for cleaning laundry and crockery.